[1]童欣怡,李琦,陈文倩,等.杨木屑木聚糖碱法提取及其制备低聚木糖的工艺研究[J].林业工程学报,2020,5(01):61-68.[doi:10.13360/j.issn.2096-1359.201904030]
 TONG Xinyi,LI Qi,CHEN Wenqian,et al.Alkali extraction of xylan from poplar sawdust and preparation of xylooligosaccharide by enzymatic hydrolysis[J].Journal of Forestry Engineering,2020,5(01):61-68.[doi:10.13360/j.issn.2096-1359.201904030]
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杨木屑木聚糖碱法提取及其制备低聚木糖的工艺研究()
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《林业工程学报》[ISSN:1001-8081/CN:32-1160/S]

卷:
5
期数:
2020年01期
页码:
61-68
栏目:
林产化学加工
出版日期:
2020-01-07

文章信息/Info

Title:
Alkali extraction of xylan from poplar sawdust and preparation of xylooligosaccharide by enzymatic hydrolysis
文章编号:
2096-1359(2020)01-0061-08
作者:
童欣怡1李琦12陈文倩1赵林果12*
1.南京林业大学化学工程学院,南京 210037; 2. 南京林业大学林业资源高效加工利用协同创新中心,南京 210037
Author(s):
TONG Xinyi1 LI Qi12 CHEN Wenqian1 ZHAO Linguo12*
1. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China; 2. Jiangsu Co-Innovation Center for Efficient Processing and Utilization of Forest Products, Nanjing Forestry University, Nanjing 210037, China
关键词:
杨木屑 木聚糖 碱法提取 正交法 条件优化
Keywords:
poplar sawdust xylan alkaline extraction orthogonal method condition optimization
分类号:
TQ351.3
DOI:
10.13360/j.issn.2096-1359.201904030
文献标志码:
A
摘要:
为充分利用杨树资源,以杨木加工废弃物杨木屑为原料,研究碱法提取木聚糖的工艺条件,并采取酶法制备低聚木糖。以质量分数为1%的稀硫酸预处理可以有效提高杨木屑木聚糖的得率,较对照组提高了2倍。对比3种碱液(NaOH、KOH和NaHCO3)提取杨木屑木聚糖的得率,以NaOH提取的木聚糖得率最大。通过单因素和正交试验优化NaOH提取杨木屑木聚糖的条件,结果显示碱液质量分数10%,固液比1:10(g:mL),温度120 ℃下提取3 h所得的木聚糖得率可达到20.7%,且四因素对提取得率的影响显著程度依次为提取温度>碱液质量分数>提取时间>固液比。碱法提取杨木屑木聚糖酸水解后产物由88.69%D-木糖、4.76%纤维二糖和6.62%葡萄糖组成且不含有阿拉伯糖,说明碱法提取的杨木屑木聚糖支链上主要连有木糖。以碱法提取杨木屑木聚糖为底物,优化了来源于嗜热菌Dictyoglomus thermophilum的重组木聚糖酶XynB-DT的酶解适宜条件:在温度70 ℃,pH 6.0, 酶用量3.00 U/mL,反应时间12 h后,杨木屑木聚糖水解产物中以木二糖和木三糖为主,并含有少量木四糖,降解率达86.2%。研究结果为杨木屑木聚糖的高值化利用奠定了基础。
Abstract:
As one of the three major plantation species in China, poplar has the advantages of fast growth and high yield, which has been widely used in the wood processing industry. Poplar raw material contains 35%-45% cellulose, 20%-35% hemicellulose and 20%-25% lignin, in which, the hemicellulose could be converted into a variety of high valued-added products. Therefore, how to make full utilization of poplar resources has become the focus of current research. In order to make the complete use of poplar resources, the poplar sawdust was used as raw material to extract xylan by the alkali method to realize high value utilization of poplar resources. In this paper, conditions of the poplar sawdust xylan extraction were optimized. The results showed that the dilution pretreatment of poplar sawdust with 1% H2SO4 before extraction could effectively extract poplar sawdust xylan, which was twice higher than that of the contrast group. The yield of poplar sawdust xylan by three alkali liquids was compared. Using NaOH as alkali liquor reagent had the highest yield. Then, the extraction conditions of xylan from poplar sawdust by NaOH were optimized by the single factor and orthogonal experiments. The results showed that the yield of xylan from poplar sawdust was the highest when the NaOH mass ratio was 10%, solid-liquid ratio was 1:10,extraction temperature was 120 ℃ and the extraction time was 3 h. The four factors had significant effects on the extraction yield, which were ranked as follows: extraction temperature > alkali mass ratio > extraction time > solid-liquid ratio. The products of poplar sawdust xylan by acid hydrolysis were analyzed by HPLC. The results showed that the products by acid hydrolysis were mainly D-xylose, containing a small amount of cellobiose and glucose, and without arabinose. It was indicated that xylan was the main product of alkali extraction, and the yield of poplar sawdust xylan was up to 20.7%. In addition, 2 mg/mL poplar sawdust xylan was treated with recombinant xylanase XynB-DT from Dictyoglomus thermophilum for hydrolysis. After 12 h, under 70 ℃, pH 6.0 with the enzyme dosage of 3.00 U/mL, the final xylooligosaccharides yield was 86.2%, and the content of XOS2-3 reached to 93.9%. All these results provided a technical basis for further improving the extraction rate and quality of poplar sawdust xylan,and realizing the intensive processing of poplar sawdust. Furthermore, these offered a theoretical basis for the directional catalytic production of xylooligosaccharides by poplar sawdust.

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备注/Memo

备注/Memo:
收稿日期:2019-04-17 修回日期:2019-10-14
基金项目:“十三五”国家重点研发计划(2017YFD0601001); 南京林业大学大学生实践创新训练计划项目(2017NFUSPITP063)。
作者简介:童欣怡,女,研究方向为生物工程。通信作者:赵林果,男,教授。E-mail: lgzhao@njfu.edu.cn
更新日期/Last Update: 2019-12-10