[1]房仙颖,周江莲,王靖秋,等.桂花渣粕黄酮提取物的制备及酶法修饰[J].林业工程学报,2020,5(06):99-105.[doi:10.13360/j.issn.2096-1359.202006009]
　FANG Xianying,ZHOU Jianglian,WANG Jingqiu,et al.Preparation of flavonoids from Osmanthus dregs and enzymatic modification[J].Journal of Forestry Engineering,2020,5(06):99-105.[doi:10.13360/j.issn.2096-1359.202006009]
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桂花渣粕黄酮提取物的制备及酶法修饰()
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《林业工程学报》[ISSN:2096-1359/CN:32-1862/S]

卷:
5
期数:
2020年06期
页码:
99-105
栏目:
林产化学加工 执行主编：王飞 张美云
出版日期:
2020-11-01

文章信息/Info

Title:
Preparation of flavonoids from Osmanthus dregs and enzymatic modification
文章编号:
2096-1359（2020）06-0099-07
作者:
房仙颖1; 周江莲1; 王靖秋1; 汤锋2; 赵林果1*
1.南京林业大学化学工程学院，南京 210037；2.国际竹藤中心，北京100102
Author(s):
FANG Xianying1;  ZHOU Jianglian1;  WANG Jingqiu1;  TANG Feng2;  ZHAO Linguo1*
1. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China; 2. International Center for Bamboo and Rattan, Beijing 100102, China
关键词:
黄酮; 桂花渣粕; 生物催化; 酶法修饰; 生物活性
Keywords:
flavonoids;  Osmanthus dregs;  biocatalysis;  enzymatic modification;  bioactivity
分类号:
TQ041
DOI:
10.13360/j.issn.2096-1359.202006009
文献标志码:
A
摘要:
比较了4种桂花（金桂、银桂、丹桂和四季桂）花瓣中总黄酮的含量，选取总黄酮含量高达42.6%的金桂为研究对象，发现桂花中富含黄酮类化合物，且绝大部分残留在精油提取后的桂花渣粕中。以芦丁法测得金桂桂花渣粕中黄酮类化合物的含量高达38.7%，表明桂花渣粕具有很高的利用价值和应用开发前景。在此基础上，研究了酶法催化桂花黄酮对其成分及活性的影响。结果表明，β葡萄糖苷酶和αL鼠李糖苷酶能将桂花黄酮中的部分黄酮糖苷水解为糖基数目较少的黄酮或黄酮苷元；桂花黄酮苷中末端或者次末端为葡萄糖基的较多，几乎没有鼠李糖基；经β葡萄糖苷酶法催化后的样品在抗炎、抗氧化和抗肿瘤方面的活性显著强于未经酶催化的样品。β葡萄糖苷酶催化后的桂花黄酮对人肝癌HepG2细胞的增殖抑制率可达85%，IC50值为51.32 μg/mL，其作用机制为促进肿瘤细胞的凋亡。研究结果为桂花资源的综合高效利用提供了新思路，为制备生物活性更强的桂花黄酮提供了技术支撑。
Abstract:
Osmanthus fragrans is a traditional famous flower, which is usually used for Ornamental plant, food, essential oil production and some other fields. The essential oil of O. fragrans is widely appreciated by consumers due to its advantages of pleasure smell, relieving fatigue, analgesia, soothing mood and maintaining beauty function. Because of the low extraction yield and high price of essential oil, it would be a great waste of O. fragrans resources if the Osmanthus dregs (the essential oil extraction residue from O. fragrans) are discarded. Previous studies have shown that there are many nonvolatile components, such as flavonoids, in O. fragrans, which have a wide range of biological activities. In this paper, the efficient utilization of Osmanthus dregs, the biocatalysis of the total flavonoids from O. fragrans and the changes of the components and biological activities before and after the biocatalysis of Osmanthus flavonoids were studied. The contents of total flavonoids in the petals of four O. fragrans were compared and the ‘Jingui’ was selected as a raw material. It was found that O. fragrans is rich in flavonoids, the content of which is up to 42.6% in ‘Jingui’ petals, and most of them remain in the dregs. The content of flavonoids in Osmanthus dregs was up to 38.7% determined by the rutin method, which indicated that the dregs of O. fragrans had high utilization value and development prospect. Natural flavonoids mostly exist in the form of glycosides, so are the flavonoids from O. fragrans. Studies have shown that the reduction in the amount of Oglycosylation can improve the antioxidation, antidiabetic, antiinflammatory, antibacterial and antitumor activities of flavonoids. The major glycosides in Osmanthus flavonoids are glucoside and a small amount of rhamnoside. On this basis, the effects of enzymatic catalysis of Osmanthus flavonoids on its components and activities were studied. Results showed that part of the flavonoid glycosides in O. fragrans can be hydrolyzed to flavonoids with less glycosyl number or flavonoid aglycone by βglucosidase and αLrhamnosidase. There were more glucosyl groups in the terminal or secondary terminal of the Osmanthus flavonoid glycosides, but little rhamnosyl. The antiinflammatory, antioxidation and antitumor activities of the samples transformed by βglucosidase were significantly higher than that of the samples being not transformed by βglucosidase. The inhibition rate on the proliferation of human hepatoma HepG2 cells of Osmanthus flavonoids transformed by βglucosidase was up to 85%, the IC50 value was 51.32 μg/mL, and the mechanism of which was promoting the apoptosis of tumor cells. The research data can provide a new idea for the comprehensive and efficient utilization of Osmanthus resources and provide technical support for the preparation of Osmanthus flavonoids with higher biological activities.

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备注/Memo

备注/Memo:
收稿日期：2020-06-08? ? ?修回日期：2020-09-21
基金项目：林业公益性行业科研专项项目（201404601）。
作者简介：房仙颖，女，讲师，研究方向为天然活性物质开发。通信作者:赵林果，男，教授。E-mail: njfu2304@163.com
更新日期/Last Update: 2020-11-11